Understanding the Canine Nuclear Factor Kappa B p65 (NF-κB p65) ELISA Kit

The Canine Nuclear Factor Kappa B p65 (NF-κB p65) ELISA Kit is a critical tool for researchers studying the intricate pathways of inflammation and immune response in dogs. As NF-κB p65 plays a pivotal role in regulating immune and inflammatory responses, having reliable and precise methods to quantify this protein is essential for advancing veterinary medicine and comparative biomedical research.

What is NF-κB p65?

Nuclear Factor Kappa B (NF-κB) is a family of transcription factors that are essential regulators of immune and inflammatory responses, cellular growth, and apoptosis. The p65 subunit, also known as RelA, is a crucial component of the NF-κB complex. It is involved in the activation of target genes that mediate immune responses to infections and stress signals.

For more detailed information on the biological role of NF-κB p65, refer to NIH resources and National Library of Medicine.

Importance of Studying NF-κB p65 in Canines

Dogs serve as significant models for understanding human diseases, particularly in oncology, immunology, and chronic inflammation. Studying NF-κB pathways in dogs can provide insights into the progression of diseases such as cancer, arthritis, and inflammatory bowel disease. Research supported by institutions like the National Cancer Institute and the Centers for Disease Control and Prevention highlights the translational potential of canine studies for human health.

Applications of the NF-κB p65 ELISA Kit

  1. Inflammatory Research: This kit is used to measure NF-κB p65 levels in various canine samples, helping to identify molecular mechanisms underlying inflammation.
  2. Cancer Studies: Aberrant NF-κB activity is often linked to cancer. Monitoring p65 expression levels aids in understanding tumor biology.
  3. Veterinary Medicine: This kit supports diagnostic and therapeutic advancements in veterinary practices, ensuring the health and well-being of dogs.
  4. Immunological Studies: By tracking NF-κB p65 activity, researchers can better understand autoimmune diseases and the efficacy of immunomodulatory treatments in canines.

Features of the Canine NF-κB p65 ELISA Kit

  • High Sensitivity and Specificity: Designed to detect low levels of NF-κB p65 with minimal cross-reactivity.
  • Ease of Use: User-friendly protocols make it accessible for researchers and clinicians.
  • Reliable Results: Stringent quality controls ensure reproducibility.
  • Broad Sample Compatibility: Suitable for serum, plasma, and tissue lysates.

Protocol and Sample Preparation

The kit includes a detailed protocol for preparing samples such as serum, plasma, or tissue lysates. Accurate sample handling is crucial to avoid variability in results. For best practices in laboratory protocols, consult CDC Laboratory Guidelines.

Advancing Research with Trusted Resources

The availability of robust tools like the NF-κB p65 ELISA Kit enables researchers to uncover critical pathways in canine health. Collaborative efforts supported by government and academic institutions, such as Grants.gov and NSF.gov, further advance this field.

Potential Future Applications

  1. Therapeutic Development: Targeting NF-κB p65 pathways for drug development could revolutionize treatments for chronic diseases in canines.
  2. Precision Medicine: Personalized approaches to veterinary care could utilize NF-κB p65 measurements to tailor treatments.
  3. Comparative Studies: Bridging veterinary and human medicine through comparative research could enhance understanding of diseases common to both species.

Conclusion

The Canine Nuclear Factor Kappa B p65 ELISA Kit is an indispensable resource for understanding the molecular underpinnings of immune response and disease in dogs. By leveraging this tool, researchers can contribute to breakthroughs in both veterinary and human medicine. For comprehensive guidelines and technical support, consider accessing educational resources from PubMed and National Institutes of Health.

Incorporating such advanced methodologies will undoubtedly propel veterinary science toward a future of precision medicine and improved animal welfare. For ongoing updates and standards, consult trusted government resources like FDA Animal & Veterinary.

For further inquiries about the kit’s specifications and applications, visit the USDA Animal Health Research.

 


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